Danmu video sharing sites are most popular social platforms for animations audience. This study traces audiences reactions to Japanese animation personas through the theoretical framework of parasocial interaction. A thematic analysis using content analysis of 293 overlaid comments on Bilibili.tv is conducted. Results indicate that audiences’ attitude tendency and length of comments have significant difference between types of personas in Hero-Villain Japanese animation. Results also indicate a positive relationship between attitude tendency of personas and length of comments and significant correlation between parasocial interaction expression types and length of comments. This study measures the immediate-PSI and its findings support the view of liner progression of parasocial interaction and parasocial relationships. In addition, this study testes the effect of audience interaction on audience expressions by instrumental variable method and advises some directions for further studies.